Long shot but anyone 1 biomdical scientist in here? - HotUKDeals
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Long shot but anyone 1 biomdical scientist in here?

shosie Avatar
6y, 8m agoPosted 6 years, 8 months ago
Need help with something
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shosie Avatar
6y, 8m agoPosted 6 years, 8 months ago
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#1
Fire away! Btw, I am not a Biomedical scientist and won't be able to answer your question but I'm still interested!
#2
ei8hty5ive
Fire away! Btw, I am not a Biomedical scientist and won't be able to answer your question but I'm still interested!


I need help analysing a pateint plasma protein sample after its gone electrophoresis
#3
shosie
I need help analysing a pateint plasma protein sample after its gone electrophoresis


http://i44.tinypic.com/2di3l0x.jpg
#4
Am a biochemist, might be able to help.
#5
shosie
I need help analysing a pateint plasma protein sample after its gone electrophoresis


i was gonna guess that was what you were gonna ask......:oops:
#6
Civic EG6
Am a biochemist, might be able to help.


well it is clinical biochemistry related
#7
tell me the question
#8
Civic EG6
tell me the question


what is paraproteinaemia and what other tests would u run to confirm it sept froma thick band on EPH
#9
Can't help with the EPH part but I think paraproteinaemia is when their is an increased amount of gamma globulin in the blood plasma.
#10
Civic EG6
Can't help with the EPH part but I think paraproteinaemia is when their is an increased amount of gamma globulin in the blood plasma.


thats as much as ive got lol. how else would u diagnose
1 Like #11
Hi I am a google-ologist:
Paraproteinaemia

A paraprotein is a monoclonal immunoglobulin or light chain present in the blood or urine; it is produced by a clonal population of mature B cells, most commonly plasma cells.[SIZE=2]1[/SIZE] Paraproteinaemia represents a group of related diseases characterised by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin. Plasma cell disorders can be considered as a spectrum of conditions ranging from monoclonal gammopathy of undetermined significance (MGUS), through asymptomatic, to symptomatic myeloma.[SIZE=2]1[/SIZE]

Epidemiology In individuals aged over 50 years the incidence of a paraprotein is 3.2%.

Causes[SIZE=2]2[/SIZE]
[LIST]
[*]Artefacts: heparinised blood sample
[*]Monoclonal gammopathy of undetermined significance (MGUS):[LIST]
[*]MGUS is defined by a low level of paraprotein <30 g/l, bone marrow plasma cells <10% and the absence of myeloma related organ or tissue damage (predominantly renal, skeletal or bone marrow impairment).
[*]Patients are often elderly and in good health.
[*]MGUS requires no therapy and the overall risk of progression to myeloma is 1% per year.[SIZE=2]1[/SIZE]
[*]Follow-up must be continued indefinitely because multiple myeloma, amyloidosis, macroglobulinaemia or related disorders may occur.[SIZE=2]3[/SIZE][/LIST]
[*]Haematological:
[LIST]
[*]Cold agglutinin disease: IgM paraprotein
[*]Paroxysmal cold haemoglobinuria: Donnath-Landsteiner antibody
[*]Warm antibody haemolytic anaemia[/LIST]
[*]Malignant neoplastic conditions:
[LIST]
[*]Heavy chain diseases: there are 3 variants - gamma, alpha and mu heavy chain disease. The alpha variant is most common, occurring particularly in people from the Mediterranean and Middle East. Often present with weakness, fatigue and fever.
[*]Waldenstrom's macroglobulinemia: Clinically similar to multiple myeloma, lymphoma and chronic lymphatic leukaemia. Often presents with non-specific weakness and fatigue but any system can be involved. Diagnosis is by serum electrophoresis and bone marrow aspiration. Treatment of symptomatic patients includes chemotherapy and supportive therapy aimed at the correction of anaemia and hyperviscosity.
[*]Chronic lymphocytic leukaemia.
[*]Myeloma:[SIZE=2]1[/SIZE][LIST]
[*]Myeloma remains incurable with a median survival of 3-4 years.
[*]Autologous stem cell transplant can prolong survival.
[*]Thalidomide in combination with dexamethasone has an emerging role in the treatment of myeloma.[/LIST]
[*]Non-Hodgkin's lymphoma
[*]POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes)[/LIST]
[*]Non-malignant systemic disease:[SIZE=2]1[/SIZE]
[LIST]
[*]Autoimmune disease: rheumatoid arthritis, scleroderma, Hashimoto thyroiditis
[*]Cutaneous disease: pyoderma gangrenosum, necrobiotic xanthogranulomatosis
[*]Liver disease: hepatitis, cirrhosis
[*]Infectious disease: tuberculosis, bacterial endocarditis[/LIST]
[*]Miscellaneous syndromes:
[LIST]
[*]Schnitzler syndrome (chronic, non-pruritic urticaria associated with recurrent fever, bone pain, arthralgia or arthritis, and a monoclonal IgM gammopathy)[/LIST]
[/LIST]Presentation
[LIST]
[*]May be discovered incidentally.
[*]Often non-specific presentation with fever, malaise, bone pain.
[*]Clinical indications for screening for M-protein:[SIZE=2]1[/SIZE][LIST]
[*]Malaise and fatigue
[*]Bone disease (persistent back pain, osteopenia or lytic lesions)
[*]Impaired renal function
[*]Normochromic normocytic anaemia ± pancytopenia
[*]Hypercalcaemia
[*]Recurrent bacterial infections
[*]Hyperviscosity
[*]Nephrotic syndrome, cardiac failure, malabsorption
[*]Peripheral neuropathies, carpal tunnel syndrome
[*]Incidental persistent elevated erythrocyte sedimentation rate[/LIST]
[/LIST]Investigations The differentiation of benign paraproteinaemia from neoplastic states is based on the absence of bone marrow disease, a relatively low and constant concentration of serum paraprotein, the absence of urine light chain excretion and normal levels of other serum immunoglobulins.
[LIST]
[*]Serum protein electrophoresis showing M-protein:[SIZE=2]4[/SIZE] Total protein and protein electrophoresis with paraprotein quantification, paraprotein typing, immunoglobulins G, A, M; beta-2-microglobulin.
[*]Full blood count, blood film, ESR: underlying cause, e.g. hypercalcaemia, high total protein, and high ESR in patients with myeloma.
[*]Urine protein, Bence Jones protein.
[*]Bone marrow aspiration and trephine biopsy.[/LIST]
#13
wow! anyone here feeling er.....dumb!

brb going to go back to school and listen this time!
#14
lol at Bargain surfer - the GOOGLEOLIGIST!!!
#15
bossyboots
lol at Bargain surfer - the GOOGLEOLIGIST!!!


+1 LOL
#16
IS EPH short hand for electrophoresis?

shosie
thats as much as ive got lol. how else would u diagnose
#17
Civic EG6
IS EPH short hand for electrophoresis?


yep it is
#18
repped all helpful answers
#19
bargain surfer;8115283

Presentation
[LIST]
[*]May be discovered incidentally.
[*]Often non-specific presentation with fever, malaise, bone pain.
[*]Clinical indications for screening for M-protein:[SIZE=2]1[/SIZE][LIST]
[*]Malaise and fatigue
[*]Bone disease (persistent back pain, osteopenia or lytic lesions)
[*]Impaired renal function
[*]Normochromic normocytic anaemia ± pancytopenia
[*]Hypercalcaemia
[*]Recurrent bacterial infections
[*]Hyperviscosity
[*]Nephrotic syndrome, cardiac failure, malabsorption
[*]Peripheral neuropathies, carpal tunnel syndrome
[*]Incidental persistent elevated erythrocyte sedimentation rate[/LIST]
[/LIST]Investigations The differentiation of benign paraproteinaemia from neoplastic states is based on the absence of bone marrow disease, a relatively low and constant concentration of serum paraprotein, the absence of urine light chain excretion and normal levels of other serum immunoglobulins.
[LIST]
[*]Serum protein electrophoresis showing M-protein: Total protein and protein electrophoresis with paraprotein quantification, paraprotein typing, immunoglobulins G, A, M; beta-2-microglobulin.
[*]Full blood count, blood film, ESR: underlying cause, e.g. hypercalcaemia, high total protein, and high ESR in patients with myeloma.
[*]Urine protein, Bence Jones protein.
[*]Bone marrow aspiration and trephine biopsy.[/LIST]


bossyboots;8115293
lol at Bargain surfer - the GOOGLEOLIGIST!!!


Foxy102;8115313
+1 LOL

Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:)
#20
bargain surfer
Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:)


if you dont say so urself
#21
bargain surfer;8115403
Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:)


shosie;8115450
if you dont say so urself

Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:) If I don't say so myself.:-D

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