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    Long shot but anyone 1 biomdical scientist in here?

    Need help with something

    21 Comments

    Fire away! Btw, I am not a Biomedical scientist and won't be able to answer your question but I'm still interested!

    Original Poster

    ei8hty5ive;8114738

    Fire away! Btw, I am not a Biomedical scientist and won't be able to … Fire away! Btw, I am not a Biomedical scientist and won't be able to answer your question but I'm still interested!



    I need help analysing a pateint plasma protein sample after its gone electrophoresis

    shosie;8114760

    I need help analysing a pateint plasma protein sample after its gone … I need help analysing a pateint plasma protein sample after its gone electrophoresis



    http://i44.tinypic.com/2di3l0x.jpg

    Am a biochemist, might be able to help.

    shosie;8114760

    I need help analysing a pateint plasma protein sample after its gone … I need help analysing a pateint plasma protein sample after its gone electrophoresis



    i was gonna guess that was what you were gonna ask......:oops:

    Original Poster

    Civic EG6;8114816

    Am a biochemist, might be able to help.



    well it is clinical biochemistry related

    tell me the question

    Original Poster

    Civic EG6;8115099

    tell me the question



    what is paraproteinaemia and what other tests would u run to confirm it sept froma thick band on EPH

    Can't help with the EPH part but I think paraproteinaemia is when their is an increased amount of gamma globulin in the blood plasma.

    Original Poster

    Civic EG6;8115260

    Can't help with the EPH part but I think paraproteinaemia is when their … Can't help with the EPH part but I think paraproteinaemia is when their is an increased amount of gamma globulin in the blood plasma.



    thats as much as ive got lol. how else would u diagnose

    Hi I am a google-ologist:
    Paraproteinaemia

    A paraprotein is a monoclonal immunoglobulin or light chain present in the blood or urine; it is produced by a clonal population of mature B cells, most commonly plasma cells.][SIZE=2]1[/SIZE] Paraproteinaemia represents a group of related diseases characterised by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin. Plasma cell disorders can be considered as a spectrum of conditions ranging from ]monoclonal gammopathy of undetermined significance (MGUS), through asymptomatic, to symptomatic ]myeloma.][SIZE=2]1[/SIZE]

    Epidemiology In individuals aged over 50 years the incidence of a paraprotein is 3.2%.

    Causes][SIZE=2]2[/SIZE]
    [LIST]
    [*]Artefacts: heparinised blood sample
    [*]Monoclonal gammopathy of undetermined significance (MGUS):[LIST]
    [*]MGUS is defined by a low level of paraprotein ]bone marrow plasma cells [*]Patients are often elderly and in good health.
    [*]MGUS requires no therapy and the overall risk of progression to myeloma is 1% per year.][SIZE=2]1[/SIZE]
    [*]Follow-up must be continued indefinitely because multiple myeloma, ]amyloidosis, macroglobulinaemia or related disorders may occur.][SIZE=2]3[/SIZE][/LIST]
    [*]Haematological:
    [LIST]
    [*]Cold agglutinin disease: IgM paraprotein
    [*]]Paroxysmal cold haemoglobinuria: Donnath-Landsteiner antibody
    [*]Warm antibody ]haemolytic anaemia[/LIST]
    [*]Malignant neoplastic conditions:
    [LIST]
    [*]Heavy chain diseases: there are 3 variants - gamma, alpha and mu ]heavy chain disease. The alpha variant is most common, occurring particularly in people from the Mediterranean and Middle East. Often present with weakness, fatigue and fever.
    [*]Waldenstrom's macroglobulinemia: Clinically similar to multiple myeloma, lymphoma and chronic lymphatic leukaemia. Often presents with non-specific weakness and fatigue but any system can be involved. Diagnosis is by serum electrophoresis and bone marrow aspiration. Treatment of symptomatic patients includes chemotherapy and supportive therapy aimed at the correction of anaemia and hyperviscosity.
    [*]]Chronic lymphocytic leukaemia.
    [*]Myeloma:][SIZE=2]1[/SIZE][LIST]
    [*]Myeloma remains incurable with a median survival of 3-4 years.
    [*]Autologous stem cell transplant can prolong survival.
    [*]Thalidomide in combination with dexamethasone has an emerging role in the treatment of myeloma.[/LIST]
    [*]S LYMPHOMA&MaxResults=50"]Non-Hodgkin's lymphoma
    [*]]POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes)[/LIST]
    [*]Non-malignant systemic disease:][SIZE=2]1[/SIZE]
    [LIST]
    [*]Autoimmune disease: ]rheumatoid arthritis, ]scleroderma, ]Hashimoto thyroiditis
    [*]Cutaneous disease: ]pyoderma gangrenosum, necrobiotic xanthogranulomatosis
    [*]Liver disease: ]hepatitis, ]cirrhosis
    [*]Infectious disease: ]tuberculosis, ]bacterial endocarditis[/LIST]
    [*]Miscellaneous syndromes:
    [LIST]
    [*]Schnitzler syndrome (chronic, non-pruritic urticaria associated with recurrent fever, ]bone pain, arthralgia or arthritis, and a monoclonal IgM gammopathy)[/LIST]
    [/LIST]Presentation
    [LIST]
    [*]May be discovered incidentally.
    [*]Often non-specific presentation with fever, malaise, bone pain.
    [*]Clinical indications for screening for M-protein:][SIZE=2]1[/SIZE][LIST]
    [*]Malaise and fatigue
    [*]Bone disease (persistent ]back pain, ]osteopenia or lytic lesions)
    [*]]Impaired renal function
    [*]Normochromic normocytic anaemia ± pancytopenia
    [*]]Hypercalcaemia
    [*]Recurrent bacterial infections
    [*]Hyperviscosity
    [*]]Nephrotic syndrome, ]cardiac failure, ]malabsorption
    [*]Peripheral neuropathies, ]carpal tunnel syndrome
    [*]Incidental persistent elevated ]erythrocyte sedimentation rate[/LIST]
    [/LIST]Investigations The differentiation of ]benign paraproteinaemia from neoplastic states is based on the absence of bone marrow disease, a relatively low and constant concentration of serum paraprotein, the absence of urine light chain excretion and normal levels of other serum immunoglobulins.
    [LIST]
    [*]]Serum protein electrophoresis showing M-protein:][SIZE=2]4[/SIZE] Total protein and protein electrophoresis with paraprotein quantification, paraprotein typing, immunoglobulins G, A, M; beta-2-microglobulin.
    [*]Full blood count, blood film, ESR: underlying cause, e.g. hypercalcaemia, high total protein, and high ESR in patients with myeloma.
    [*]Urine protein, Bence Jones protein.
    [*]Bone marrow aspiration and trephine biopsy.[/LIST]

    wow! anyone here feeling er.....dumb!

    brb going to go back to school and listen this time!

    lol at Bargain surfer - the GOOGLEOLIGIST!!!

    bossyboots;8115293

    lol at Bargain surfer - the GOOGLEOLIGIST!!!



    +1 LOL

    IS EPH short hand for electrophoresis?

    shosie;8115275

    thats as much as ive got lol. how else would u diagnose

    Original Poster

    Civic EG6;8115322

    IS EPH short hand for electrophoresis?



    yep it is

    Original Poster

    repped all helpful answers

    bargain surfer;8115283

    Presentation[LIST][*]May be discovered incidentally.[*]Often non-specific … Presentation[LIST][*]May be discovered incidentally.[*]Often non-specific presentation with fever, malaise, bone pain.[*]Clinical indications for screening for M-protein:][SIZE=2]1[/SIZE][LIST][*]Malaise and fatigue[*]Bone disease (persistent back pain, osteopenia or lytic lesions)[*]Impaired renal function[*]Normochromic normocytic anaemia ± pancytopenia[*]Hypercalcaemia[*]Recurrent bacterial infections[*]Hyperviscosity[*]Nephrotic syndrome, cardiac failure, malabsorption[*]Peripheral neuropathies, carpal tunnel syndrome[*]Incidental persistent elevated erythrocyte sedimentation rate[/LIST][/LIST]Investigations The differentiation of benign paraproteinaemia from neoplastic states is based on the absence of bone marrow disease, a relatively low and constant concentration of serum paraprotein, the absence of urine light chain excretion and normal levels of other serum immunoglobulins.[LIST][*]Serum protein electrophoresis showing M-protein: Total protein and protein electrophoresis with paraprotein quantification, paraprotein typing, immunoglobulins G, A, M; beta-2-microglobulin.[*]Full blood count, blood film, ESR: underlying cause, e.g. hypercalcaemia, high total protein, and high ESR in patients with myeloma.[*]Urine protein, Bence Jones protein.[*]Bone marrow aspiration and trephine biopsy.[/LIST]



    bossyboots;8115293

    lol at Bargain surfer - the GOOGLEOLIGIST!!!



    Foxy102;8115313

    +1 LOL


    Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:)

    Original Poster

    bargain surfer;8115403

    Actually more of a Geneticist tbh, but I has done extremely well in … Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:)



    if you dont say so urself

    bargain surfer;8115403

    Actually more of a Geneticist tbh, but I has done extremely well in … Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:)



    shosie;8115450

    if you dont say so urself


    Actually more of a Geneticist tbh, but I has done extremely well in Googleology also:) If I don't say so myself.:-D
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